Selected publications from the facility and its users:

Ali, T., Coles, P., Stevens, T. J., Stott, K. & Thomas, J. O. (2004). Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p. J. Mol. Biol. 338, 139-48.

Assenberg, R., Webb, M., Connolly, E., Stott, K., Watson, M., Hobbs, J. and Thomas, J. O. (2008). A critical role in structure-specific DNA binding for the acetylatable lysine residues in HMGB1. Biochem. J.411, 553-561.

Bailey, L. K., Campbell, L. J., Evetts, K. A., Littlefield, K., Rajendra, E., Nietlispach, D., Owen, D. and Mott, H. R. (2009). The Structure of Binder of Arl2 (BART) Reveals a Novel G Protein Binding Domain IMPLICATIONS FOR FUNCTION. J. Biol. Chem. 284, 992-999.

Bois, P. R. J., O’Hara, B. P., Nietlispach, D., Kirkpatrick, J. and Izard, T. (2006). The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin. J. Biol. Chem. 281, 7228-7236.

Bolton, D., Evans, P. A., Stott, K. and Broadhurst, R. W. (2001). Structure and properties of a dimeric N-terminal fragment of human ubiquitin. J. Mol. Biol. 314, 773-787.

Brasher, S. V., Smith, B. O., Fogh, R. H., Nietlispach, D., Thiru, A., Nielsen, P. R., Broadhurst, R. W., Ball, L. J., Murzina, N. V. and Laue, E. D. (2000). The structure of mouse HP1 suggests a unique mode of single peptide recognition by the shadow chrome domain dimer. EMBO J. 19, 1587-1597.

Broadhurst, R. W., Nietlispach, D., Wheatcroft, M. P., Leadlay, P. F. and Weissman, K. J. (2003). The structure of docking domains in modular polyketide synthases. Chem. Biol. 10, 723-731.

Cato, L., Stott, K., Watson, M. and Thomas, J. O. (2008). The Interaction of HMGB1 and Linker Histones Occurs Through their Acidic and Basic Tails. J. Mol. Biol. 384, 1262-1272.

Chattopadhyay, A., Jones, N. G., Nietlispach, D., Nielsen, P. R., Voorheis, H. P., Mott, H. R. and Carrington, M. (2005). Structure of the C-terminal domain from Trypanosoma brucei variant surface glycoprotein MITat1.2. J. Biol. Chem. 280, 7228-7235.

Cheung, M. S., Maguire, M. L., Stevens, T. J. and Broadhurst, R. W. (2010). DANGLE: A Bayesian inferential method for predicting protein backbone dihedral angles and secondary structure. J. Magn. Reson. 202, 223-233.

Dunne, A., Ejdeback, M., Ludidi, P. L., O’Neill, L. A. J. and Gay, N. J. (2003). Structural complementarity of Toll/interleukin-1 receptor domains in toll-like receptors and the adaptors Mal and MyD88. J. Biol. Chem. 278, 41443-41451.

Fenwick, R. B., Prasannan, S., Campbell, L. J., Nietlispach, D., Evetts, K. A., Camonis, J., Mott, H. R. and Owen, D. (2009). Solution Structure and Dynamics of the Small GTPase RalB in Its Active Conformation: Significance for Effector Protein Binding. Biochemistry 48, 2192-2206.

Fogh, R. H., Boucher, W., Vranken, W. F., Pajon, A., Stevens, T. J., Bhat, T. N., Westbrook, J., Ionides, J. M. C. and Laue, E. D. (2005). A framework for scientific data modeling and automated software development. Bioinformatics 21, 1678-1684.

Fogh, R. H., Vranken, W. F., Boucher, W., Stevens, T. J. and Laue, E. D. (2006). A nomenclature and data model to describe NMR experiments. J. Biomol. NMR 36, 147-155.

Gangloff, M., Murali, A., Xiong, J., Arnot, C. J., Weber, A. N., Sandercock, A. M., Robinson, C. V., Sarisky, R., Holzenburg, A., Kao, C. and Gay, N. J. (2008). Structural insight into the mechanism of activation of the Toll receptor by the dimeric ligand Spatzle. J. Biol. Chem. 283, 14629-14635.

Gautier, A., Kirkpatrick, J. P. and Nietlispach, D. (2008). Solution-state NMR spectroscopy of a seven-helix transmembrane protein receptor: Backbone assignment, secondary structure, and dynamics. Angew. Chem. Int.. Edit. 47, 7297-7300.

Gautier, A., Mott, H. R., Bostock, M. J., Kirkpatrick, J. P. and Nietlispach, D. (2010). Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy. Nat. Struct. Mol. Biol. 17, 768-774.

Grimshaw, S. J., Mott, H. R., Stott, K. M., Nielsen, P. R., Evetts, K. A., Hopkins, L. J., Nietlispach, D. and Owen, D. (2004). Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM. J. Biol. Chem. 279, 2192-2201.

Ikeya, T., Sasaki, A., Sakakibara, D., Shigemitsu, Y., Hamatsu, J., Hanashima, T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., Nietlispach, D., Walchli, M., Smith, B. O., Shirakawa, M., Guntert, P. and Ito, Y. (2010). NMR protein structure determination in living E. coli cells using nonlinear sampling. Nat. Protoc. 5, 1051-1060.

Jansma, A. L., Kirkpatrick, J. P., Hsu, A. R., Handel, T. M. and Nietlispach, D. (2010). NMR Analysis of the Structure, Dynamics, and Unique Oligomerization Properties of the Chemokine CCL27. J. Biol. Chem. 285, 14424-14437.

Jones, N. G., Nietlispach, D., Sharma, R., Burke, D. F., Eyres, I., Mues, M., Mott, H. R. and Carrington, M. (2008). Structure of a glycosylphosphatidylinositol-anchored domain from a trypanosome variant surface glycoprotein. J. Biol. Chem. 283, 3584-3593.

Khan, F., Stott, K. & Jackson, S. (2003). 1H, 15N and 13C backbone assignment of the green fluorescent protein (GFP). J. Biomol. NMR, 26, 281-2.

Kolocouris, A., Hansen, R. K. and Broadhurst, R. W. (2004). Interaction between an amantadine analogue and the transmembrane portion of the influenza A M2 protein in liposomes probed by H-1 NMR spectroscopy of the ligand. J. Med. Chem. 47, 4975-4978.

Lengyel, J. S., Stott, K. M., Wu, X., Brooks, B. R., Balbo, A., Schuck, P., Perham, R. N., Subramaniam, S. & Milne, J. L. (2008). Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex. Structure, 16, 93-103.

Lochner, M. and Lummis, S. C. R. (2010). Agonists and Antagonists Bind to an A-A Interface in the Heteromeric 5-HT(3)AB Receptor. Biophys. J. 98, 1494-1502.

Lummis, S. C. R., Beene, D. L., Lee, L. W., Lester, H. A., Broadhurst, R. W. and Dougherty, D. A. (2005). Cis-trans isomerization at a proline opens the pore of a neurotransmitter-gated ion channel. Nature 438, 248-252.

Maguire, M. L., Guler-Gane, G., Nietlispach, D., Raine, A. R. C., Zorn, A. M., Standart, N. and Broadhrust, R. W. (2005). Solution structure and backbone dynamics of the KH-QUA2 region of the Xenopus STAR/GSG quaking protein. J. Mol. Biol. 348, 265-279.

Main, E. R., Stott, K., Jackson, S. E. & Regan, L. (2005). Local and long-range stability in tandemly arrayed tetratricopeptide repeats. Proc. Natl. Acad. Sci., 102, 5721-6.

Modha, R., Campbell, L. J., Nietlispach, D., Buhecha, H. R., Owen, D. and Mott, H. R. (2008). The Rac1 polybasic region is required for interaction with its effector PRK1. J. Biol. Chem.283, 1492-1500.

Moncrieffe, M. C., Stott, K. M. and Gay, N. J. (2005). Solution structure of the isolated Pelle death domain. FEBS Lett. 579, 3920-3926.

Moncrieffe, M. C., Grossmann, J. G. and Gay, N. J. (2008). Assembly of Oligomeric Death Domain Complexes during Toll Receptor Signaling. J. Biol. Chem. 283, 33447-33454.

Monie, T. P., Moncrieffe, M. C. and Gay, N. J. (2009). Structure and regulation of cytoplasmic adapter proteins involved in innate immune signaling. Immunol. Rev. 227, 161-175.

Morreale, A., Venkatesan, M., Mott, H. R., Owen, D., Nietlispach, D., Lowe, P. N. and Laue, E. D. (2000). Structure of Cdc42 bound to the GTPase binding domain of PAK. Nat. Struct. Biol. 7, 384-388.

Motshwene, P. G., Moncrieffe, M. C., Grossmann, J. G., Kao, C., Ayaluru, M., Sandercock, A. M., Robinson, C. V., Latz, E. and Gay, N. J. (2009). An Oligomeric Signaling Platform Formed by the Toll-like Receptor Signal Transducers MyD88 and IRAK-4. J. Biol. Chem. 284, 25404-25411.

Mott, H. R., Owen, D., Nietlispach, D., Lowe, P. N., Manser, E., Lim, L. and Laue, E. D. (1999). Structure of the small G protein Cdc42 bound to the GTPase-binding domain of ACK. Nature 399, 384-388.

Mott, H. R., Nietlispach, D., Hopkins, L. J., Mirey, G., Camonis, J. H. and Owen, D. (2003). Structure of the GTPase-binding domain of Sec5 and elucidation of its Ral binding site. J. Biol. Chem. 278, 17053-17059.

Mott, H. R., Nietlispach, D., Evetts, K. A. and Owen, D. (2005). Structural analysis of the SH3 domain of beta-PIX and its interaction with alpha-p21 activated kinase (PAK). Biochemistry 44, 10977-10983.

Murzina, N. V., Pei, X. Y., Zhang, W., Sparkes, M., Vicente-Garcia, J., Pratap, J. V., McLaughlin, S. H., Ben-Shahar, T. R., Verreault, A., Luisi, B. F. and Laue, E. D. (2008). Structural basis for the recognition of histone H4 by the histone-chaperone RbAp46. Structure 16, 1077-1085.

Nagpal, K., Plantinga, T. S., Wong, J., Monks, B. G., Gay, N. J., Netea, M. G., Fitzgerald, K. A. and Golenbock, D. T. (2009). A TIR Domain Variant of MyD88 Adapter-like (Mal)/TIRAP Results in Loss of MyD88 Binding and Reduced TLR2/TLR4 Signaling. J. Biol. Chem. 284, 25742-25748.

Nielsen, P. R., Nietlispach, D., Mott, H. R., Callaghan, J., Bannister, A., Kouzarides, T., Murzin, A. G., Murzina, N. V. and Laue, E. D. (2002). Structure of the HP1 chromodomain bound to histone H3 methylated at lysine 9. Nature 416, 103-107.

Nielsen, P. R., Nietlispach, D., Buscaino, A., Warner, R. J., Akhtar, A., Murzin, A. G., Murzina, N. V. and Laue, E. D. (2005). Structure of the chromo barrel domain from the MOF acetyltransferase. J. Biol. Chem. 280, 32326-32331.

Nietlispach, D., Ito, Y. and Laue, E. D. (2002). A novel approach for the sequential backbone assignment of larger proteins: Selective intra-HNCA and DQ-HNCA. J. Am. Chem. Soc. 124, 11199-11207.

Nietlispach, D. (2005). Suppression of anti-TROSY lines in a sensitivity enhanced gradient selection TROSY scheme. J. Biomol. NMR 31, 161-166.

Owen, D., Lowe, P. N., Nietlispach, D., Brosnan, C. E., Chirgadze, D. Y., Parker, P. J., Blundell, T. L. and Mott, H. R. (2003). Molecular dissection of the interaction between the small G proteins Rac1 and RhoA and protein kinase C-related kinase 1 (PRK1). J. Biol. Chem. 278, 50578-50587.

Richter, C. D., Nietlispach, D., Broadhurst, R. W. and Weissman, K. J. (2008). Multienzyme docking in hybrid megasynthetases. Nat. Chem. Biol. 4, 75-81.

Sanderson, A., Stott, K., Stevens, T. J., Thomas, J. O. (2005). Engineering the structural stability and functional properties of the GI domain into the intrinsically unfolded GII domain of the yeast linker histone Hho1p. J. Mol. Biol. 349, 608-20.

Slatter, D. A., Foley, L. A., Peachey, A. R., Nietlispach, D. and Farndale, R. W. (2006). Rapid synthesis of a register-specific heterotrimeric type I collagen helix encompassing the integrin alpha 2 beta 1 binding site. J. Mol. Biol. 359, 289-298.

Stott, K., Watson, M., Howe, F. S., Grossmann, J. G. & Thomas. J. O. (2010). Tail-mediated collapse of HMGB1 is dynamic and occurs via differential binding of the acidic tail to the A and B domains. J. Mol. Biol., 403, 706-22.

Stott, K. M., Yusof, A. M., Perham, R. N. & Jones, D. D. (2009). A surface loop directs conformational switching of a lipoyl domain between a folded and a novel misfolded structure. Structure, 17, 1117-27.

Stott, K., Tang, G. S. F., Lee, K. B. and Thomas, J. O. (2006). Structure of a complex of tandem HMG boxes and DNA. J. Mol. Biol.360, 90-104.

Thiru, A., Nietlispach, D., Mott, H. R., Okuwaki, M., Lyon, D., Nielsen, P. R., Hirshberg, M., Verreault, A., Murzina, N. V. and Laue, E. D. (2004). Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin. Embo J. 23, 489-499.

Thompson, A. J., Lochner, M. and Lummis, S. C. R. (2008). Loop B Is a Major Structural Component of the 5-HT3 Receptor. Biophys. J. 95, 5728-5736.

Thomson, R., Molina-Portela, P., Mott, H., Carrington, M. and Raper, J. (2009). Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes. Proc. Natl. Acad. Sci. U. S. A. 106, 19509-19514.

Vranken, W. F., Boucher, W., Stevens, T. J., Fogh, R. H., Pajon, A., Llinas, P., Ulrich, E. L., Markley, J. L., Ionides, J. and Laue, E. D. (2005). The CCPN data model for NMR spectroscopy: Development of a software pipeline. Proteins 59, 687-696.

Walsh, C., Gangloff, M., Monie, T., Smyth, T., Wei, B., McKinley, T. J., Maskell, D., Gay, N. and Bryant, C. (2008). Elucidation of the MD-2/TLR4 interface required for signaling by lipid IVa. J. Immunol. 181, 1245-1254.

Watson, M., Stott, K. and Thomas, J. O. (2007). Mapping intramolecular interactions between domains in HMGB1 using a tail-truncation approach. J. Mol. Biol. 374, 1286-1297.